Definition/General

Introduction:
-Secretory carcinoma of the breast, also known as juvenile secretory carcinoma, is a very rare subtype of breast cancer
-It is characterized by the presence of intracellular and extracellular secretions
-It typically has an indolent clinical course and an excellent prognosis.
Origin:
-It is believed to arise from the terminal duct-lobular unit (TDLU)
-The tumor cells are characterized by their ability to produce and secrete a milk-like substance.
Classification:
-It is classified as a special subtype of invasive ductal carcinoma
-The diagnosis is based on its characteristic histology, which includes solid, microcystic, and glandular patterns with abundant secretions.
Epidemiology:
-It is most common in children and adolescents, hence the name "juvenile" secretory carcinoma, but it can occur at any age
-It is extremely rare, accounting for less than 0.15% of all breast cancers.

Clinical Features

Presentation:
-Typically presents as a well-circumscribed, mobile, painless mass
-It can occur in both females and males
-Nipple discharge may be present.
Symptoms:
-A slow-growing, non-tender breast lump is the most common symptom
-The mass is usually small at diagnosis.
Risk Factors:
-There are no well-defined risk factors
-Unlike other breast cancers, it is not strongly associated with hormonal factors or family history.
Screening:
-Due to its rarity and occurrence in young patients, it is usually not detected by routine screening
-Diagnosis is typically made after a palpable mass is investigated.

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Gross Description

Appearance:
-The tumor is typically well-circumscribed, firm, and lobulated
-The cut surface is gray-white and may have a mucoid or gelatinous appearance due to the secretions.
Characteristics:
-The size is usually small, often less than 3 cm
-The consistency is firm but can be soft if there is a large cystic component.
Size Location:
-Can occur anywhere in the breast, with no specific quadrant predilection.
Multifocality: Multifocality is rare.

Microscopic Description

Histological Features:
-The tumor is composed of glands and microcysts containing abundant eosinophilic, PAS-positive secretions
-The cells are arranged in solid, glandular, and papillary patterns
-The stroma is typically fibrous.
Cellular Characteristics:
-The tumor cells are bland, with uniform, round to oval nuclei, and pale to eosinophilic cytoplasm
-The cytoplasm often contains secretory vacuoles
-Mitotic activity is low.
Architectural Patterns:
-Three main patterns are described: solid, microcystic, and ductal
-A mixture of these patterns is common
-The secretions are a key diagnostic feature.
Grading Criteria: These tumors are almost always low-grade (Grade 1) due to their bland cytology and low mitotic rate.

Immunohistochemistry

Positive Markers:
-Positive for S100 protein and mammaglobin
-Often positive for ER and PR, but this can be variable
-E-cadherin is positive
-GCDFP-15 can be positive.
Negative Markers:
-Usually negative for HER2
-Myoepithelial markers are absent around the invasive components.
Diagnostic Utility:
-S100 positivity is a key feature
-The presence of the characteristic ETV6-NTRK3 gene fusion is diagnostic.
Molecular Subtypes:
-Most are considered a distinct molecular entity defined by the ETV6-NTRK3 fusion, not fitting neatly into the standard luminal/HER2/triple-negative classification.

Molecular/Genetic

Genetic Mutations:
-The pathognomonic genetic alteration is the t(12;15)(p13;q25) translocation, which results in the ETV6-NTRK3 gene fusion
-This fusion is the driving mutation.
Molecular Markers: Detection of the ETV6-NTRK3 fusion by FISH or RT-PCR is the gold standard for diagnosis.
Prognostic Significance:
-Excellent prognosis, especially in children
-The risk of lymph node metastasis is low, and distant metastasis is very rare
-Recurrence can occur after a long interval, so long-term follow-up is recommended.
Therapeutic Targets:
-The NTRK fusion is a therapeutic target
-Larotrectinib and entrectinib, which are TRK inhibitors, have shown efficacy in tumors with NTRK fusions, including secretory carcinoma.

Differential Diagnosis

Similar Entities:
-Mucinous carcinoma
-Lipid-rich carcinoma
-Acinic cell carcinoma
-Benign secretory changes in pregnancy/lactation.
Distinguishing Features:
-Mucinous carcinoma has extracellular mucin, not the eosinophilic secretions of secretory carcinoma
-Lipid-rich carcinoma has clear, vacuolated cells but lacks the ETV6-NTRK3 fusion
-Acinic cell carcinoma has granular cytoplasm but is typically S100 negative.
Diagnostic Challenges:
-The main challenge is its rarity
-Distinguishing it from other breast lesions with secretory features requires a high index of suspicion and molecular testing for the ETV6-NTRK3 fusion.
Rare Variants: The entity itself is a rare variant of breast cancer.

Sample Pathology Report

Template Format

Sample Pathology Report

Complete Report: This is an example of how the final pathology report should be structured for this condition.

Specimen Information

[specimen type], measuring [size] cm in greatest dimension

Diagnosis

[diagnosis name]

Classification

Classification: [classification system] [grade/type]

Histological Features

Shows [architectural pattern] with [nuclear features] and [mitotic activity]

Size and Extent

Size: [X] cm, extent: [local/regional/metastatic]

Margins

Margins are [involved/uninvolved] with closest margin [X] mm

Lymphovascular Invasion

Lymphovascular invasion: [present/absent]

Lymph Node Status

Lymph nodes: [X] positive out of [X] examined

Special Studies

IHC: [marker]: [result]

Molecular: [test]: [result]

[other study]: [result]

Final Diagnosis

Final diagnosis: [complete diagnosis]